Assay Plates - Chrom4

Choosing the correct microplate for your application can mean the difference between indifferent and great results. There are three basic methods of obtaining useful optical data from microplate-based samples. Absorbance assays, Fluorescence assays and Luminescence assays. Porvair offers solid bottom, clear bottom, glass bottom and UV clear bottom assay plates.

Solid Bottom Assay Plates
This range of solid bottom assay plates is specifically designed for absorbance, fluorescence, luminescence and scintillation applications.

Clear bottom assay plates
Krystal series clear bottom plates are now recognised as industry-leading tools for Pharma drug discovery and screening. These standard clear polystyrene bottom plates have bases just 0.4mm thick and are moulded from highly polished tools to reduce flow lines, artefacts and scratches, guaranteeing good results with all types of bottom-reading instruments. For the more modern demanding confocal readers, Porvair Glass Bottom plates have been shown by leading research institutes to be unsurpassed for flatness, linearity and optical clarity. The sparkling pure borosilicate glass is just 175µm thick with a flatness tolerance across the plate of +/- 15µm ensuring superb crisp images every time. We supply the Krystal clear plates treated for cell culture with a low pressure gas plasma that produces an even surface treatment right across the plate. It is particularly suited to cell lines which do not grow easily on plastics. The plastic-bottomed plates are ultrasonically welded together – a tried and tested method which both guarantees a perfect seal every time and eliminates the inconsistencies seen with laser membrane welding used by other manufacturers. Only biocompatible adhesives are used for glass bottom plates, cured by UV radiation to ensure that the finished plates are solvent-free, have very low autofluorescence and will allow good cell growth.

Glass bottom assay plates
Manufactured for whole-plate CCD imaging and laser detection applications, Krystal glass bottom plates consist of a polystyrene upper part and a clear borosilicate glass sheet fixed to the base with a proprietary adhesive. This process results in consistent flatness of the base and gives improved light transmission whilst maintaining a flat optical plane for growing cells. The nominal cut-off wavelength of 335nm allows most fluorescence assays to be excited or read through the glass bottom. All plates are supplied lidded.

  • 175µm glass thickness
  • +/- 15µm flatness across base
  • 335nm UV optical cut-off
  • Industry-standard well layout
  • Very low autofluorescence
  • High degree of planar flatness
  • Biocompatible adhesive
  • Resistant to alcohol, DMSO and PBS
  • Robot friendly
  • Manufactured to ANSI/SBS specification

UV clear bottom assay plates
Recently, scientists have begun using assay chemistries which require excitation or detection wavelengths in the far UV region, below 350nm. To enable customers to develop assays in this area, Porvair Sciences has introduced a very high specification range of COP-bottomed, UV-transparent microplates. Precision engineered and assembled, these plates are available in 96- and 384-well black matrix only for the most sensitive UV range fluorescence assays using whole-plate imaging or confocal microscopy.

  • 220nm far UV cut-off
  • 96- and 384-well black versions
  • Industry-standard well layout
  • Very low autofluorescence
  • High degree of planar flatness
  • High chemical resistance to most solvents
  • Robot friendly
  • Cyclo Olefin Polymer construction with clear base
  • Precision engineered to meet ANSI/SBS specification

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